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Finally, while our results consistently demonstrate that Se/LDH@PEG-M modulates the macrophage population by suppressing M1 markers and enhancing M2 traits, it remains to be definitively verified whether this represents a direct phenotypic reprogramming of existing M1 macrophages into M2 macrophages

We also developed methods to detect NLRP3 activation in 2D and 3D tissue using LPS, as a primer, and Nigericin, as an NLRP3 inflammasome activator [14, 35]